R&D Systems™ Recombinant Human PDGF-AA, Animal-Free Protein

Catalog No. AFL221050

Supplier: R&D Systems™ AFL221050

Description

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. ED50 for this effect is 50 to 200 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin.

Specifications

• Accession Number: P04085.1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5154
• Molecular Weight (g/mol): M.W. theroretical: 14 kDa (monomer)
• Quantity: 50 μg
• Source: E. coli-derived human PDGF-AA protein Ser87-Thr211, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Gene Alias: PDGF1, PDGF-A, PDGFAA
• Gene Symbol: PDGFA
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile 4 mM HCl.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.