R&D Systems™ Recombinant Human PDGF-AB Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 15-75 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002 ).

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
• Molecular Weight (g/mol): 14.3 kDa (A chain) & 12.3 kDa (B chain)
• Name: PDGF-AB
• Quantity: 10 μg
• Source: E. coli-derived human PDGF-AB protein, (N-terminus) Human PDGF-A (Ser87-Thr211) Accession &Num; P04085 Human PDGF-B (Ser82-Thr190) Accession &Num; P01127 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked heterodimer
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.