R&D Systems™ Recombinant Human PDGF-AB (single chain) Protein, CF

Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 2.00-20.0 ng/mL.

Specifications

• Accession Number: NP_001382295.1 & P01127.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 5154
• Molecular Weight (g/mol): MolecularWeight-observed: 28-37 kDa, under reducing conditions., MolecularWeight-theroretical: 26 kDa
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human PDGF-AB protein Human PDGFA (Ser87-Arg196) (Cys123Ala, Cys132Ala) (N-terminus) Accession # NP_001382295.1 linker Human PDGFB (Ser82-Thr190) (Cys123Ala, Cys132Ala) (N-terminus) Accession # P01127.1 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute 10 μg size at 100 μg/mL in 4 mM HCl. Reconstitute all the other sizes at 250 μg/mL in 4 mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PDGF-AB