R&D Systems™ Recombinant Human PDGF-BB, Animal-Free Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells

Supplier:  R&D Systems™ AFL220050

Catalog No. AFL220050

Description

• The ED50 for this effect is 1.5 to 6 ng/mL.
• The specific activity of Recombinant Human PDGF-BB is approximately 5.8 x 103 IU/μg, which is calibrated against Recombinant Human PDGF-BB WHO International Standard a(NIBSC code: 94/728).

Specifications

• Accession Number: P01127
• Gene ID (Entrez): 5155
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile 4 mM HCl.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): 12.3 kDa (monomer)
• Source: E. coli-derived human PDGF-BB protein Ser82-Thr190 Produced using non-animal reagents in an animal-free laboratory.
• Gene Symbol: PDGFB
• Species: Human
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.