R&D Systems™ Recombinant Human PDGF-BB Biotinylated Protein

Catalog No.	Quantity
BT220010CF	10 μg

Catalog No. BT220010CF Supplier R&D Systems™ Supplier No. BT220010/CF

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human PDGF-BB Biotinylated Protein is derived from E. coli. The Recombinant Human PDGF-BB Biotinylated Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q6FHE7
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5155
• Molecular Weight (g/mol): Observed Molecular Wt: 12kDa, reducing conditions, Predicted Molecular Wt.: 12kDa (monomer, unlabeled)
• Quantity: 10 μg
• Source: E. coli-derived human PDGF-BB protein Ser82-Thr190
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer, biotinylated protein via amines
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Common Name: PDGF-BB
• Gene Symbol: PDGFB
• Biological Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 1.5-6ng/mL.
• Conjugate: Biotin
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Ser82-Thr190
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining