R&D Systems™ Recombinant Human Pepsinogen A Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002 ). The specific activity is >7,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P00790
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 643847
• Molecular Weight (g/mol): M.W. (Observed): 40-43 kDa, reducing conditions; M.W. (theoretical): 41 kDa
• Name: Pepsinogen A
• Quality Control Testing: SDS-PAGE: 40-43kDa, reducing conditions
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human Pepsinogen A protein Met1-Ala388, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Termination: Ile16 and Val63
• Without Additives: Carrier Protein
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: PGA4
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Pepsinogen A