R&D Systems™ Recombinant Human PKLR Protein

Catalog No.	Quantity
85-69P-K050	50 μg

Catalog No. 85-69P-K050 Supplier R&D Systems™ Supplier No. 8569PK050

Description

Pyruvate Kinase Isozymes L/R

Measured by its ability to transfer phosphate from phospho(enol)pyruvic acid monosodium salt hydrate (PEP) to adenosine 5'-diphosphate sodium salt (ADP). The specific activity is >25,000 pmol/min/ug, as measured under the described conditions.

Specifications

• Accession Number: P30613
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
• Gene ID (Entrez): 5313
• Molecular Weight (g/mol): M.W. (Observed): 51-60 kDa, reducing conditions; M.W. (theoretical): 58 kDa
• Quantity: 50 μg
• Source: E. coli-derived human PKLR protein Leu47-Ser574, with N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: EC 2.7.1.40, PK1, PK1PKRL, PKL, PKR, Pyruvate kinase 1, pyruvate kinase isozyme R/L, pyruvate kinase isozymes R/L, pyruvate kinase type L, pyruvate kinase, liver and blood cell, pyruvate kinase, liver and RBC, Red cell/liver pyruvate kinase, RPK, R-type/L-type pyruvate kinase
• Common Name: PKLR
• Gene Symbol: PKLR
• Biological Activity: Measured by its ability to transfer phosphate from phospho(enol)pyruvic acid monosodium salt hydrate (PEP) to adenosine 5'-diphosphate sodium salt (ADP). The specific activity is >25,000 pmol/min/ug, as measured under the described conditions.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Leu47-Ser574, with N-terminal Met and 6-His tag
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane)
• Protein: PKLR