R&D Systems™ Recombinant Human Plexin B1 His-tag Protein

Catalog No.	Quantity
10677PB050	50 μg

Catalog No. 10677PB050 Supplier R&D Systems™ Supplier No. 10677PB050

Description

Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B1 His-Tag protein is coated at 1 μg/mL, Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4 ) binds with an ED50 of 0.02-0.12 μg/mL.

Specifications

• Accession Number: O43157.3
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 5364
• Molecular Weight (g/mol): M.W.-observed: 215-250 kDa, under reducing conditions, M.W.-theroretical: 158 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Plexin B1 protein Leu20-Gln1490 (RRRR1302-1305 AAAA), with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: plexin B1, PLXN5, PLXNB1, SEP, Transmembrane Protein SEP
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Plexin B1