R&D Systems™ Recombinant Human PlGF-4 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human PlGF-4 Protein is derived from HEK293. The Recombinant Human PlGF-4 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P49763-4
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5228
• Molecular Weight (g/mol): Observed M.W.: 35-40kDa, reducing conditions, Theoretical M.W.: 25kDa
• Quantity: 25 μg
• Source: Human embryonic kidney cell,HEK293-derived human PlGF-4 protein Leu19-Arg242
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: PlGF-4
• Gene Symbol: PGF
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 Fc Chimera, aa 27-328 is immobilized at 0.5μg/mL, 100μL/well, the concentration of Recombinant Human PlGF-4 that produces 50% of the optimal binding response is approximately 4-24ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Leu19-Arg242
• Purity or Quality Grade: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining