R&D Systems™ Recombinant Human PlGF Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 Fc Chimera (Catalog # 3516-FL ) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.75-4.5 ng/mL.

Specifications

• Accession Number: NP_001193941.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA. Reconstitute at 200μg/mL in 4 mM HCl.
• Gene ID (Entrez): 5228
• Molecular Weight (g/mol): Observed M.W.: 12-15kDa, reducing conditions, Theoretical M.W.: 15kDa
• Quantity: 10μg
• Source: E. coli-derived human PlGF protein Ala21-Arg149
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: D12S1900, PGF, PGFL, placenta growth factor, placental growth factor, placental growth factor, vascular endothelial growth factor-related protein, PlGF, PlGF-2, PLGFplacental growth factor-like, SHGC-10760
• Common Name: PLGF
• Gene Symbol: PGF
• Biological Activity: When Recombinant Human VEGF R1/Flt-1 Fc Chimera is immobilized at 0.5μg/mL, 100μL/well, the concentration of Recombinant Human PlGF that produces 50% of the optimal binding response is approximately 0.15-0.9ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Ala21-Arg149
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining