R&D Systems™ Recombinant Human PSMA/FOLH1/NAALADase I His Avi Protein

Catalog No.	Quantity
AVI4234025	25 μg

Catalog No. AVI4234025 Supplier R&D Systems™ Supplier No. AVI4234025

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Human PSMA/FOLH1 Affinity Purified Polyclonal Antibody (Catalog # AF4234 ) is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human PSMA/FOLH1/NAALADase I His-tag Avi-tag with an ED50 of 0.01-0.08 μg/mL. Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is > 250 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q04609.1
• For Use With (Application): Bioactivity, Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in MES and NaCl.
• Gene ID (Entrez): 2346
• Molecular Weight (g/mol): M.W.-observed: 94-107 kDa, under reducing conditions, M.W.-theoretical: 83 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human PSMA/FOLH1/NAALADase I protein Avi-tag HHHHHH Human PSMA/FOLH1/NAALADase I (Lys44-Ala750) (N-terminus) Accession # Q04609.1 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Cell growth-inhibiting gene 27 protein, cell growth-inhibiting protein 27, EC 3.4.17.21, FGCP, folate hydrolase (prostate-specific membrane antigen) 1, Folate hydrolase 1, FOLH1, FOLHNAALADase I, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, GCPII, GCPIImGCP, glutamate carboxylase II, glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, mopsm, NAALAD1, NAALAD1N-acetylated-alpha-linked acidic dipeptidase I, NAALAdase, NAALADase I, N-acetylated alpha-linked acidic dipeptidase 1, prostate specific membrane antigen variant F, Prostate-specific membrane antigen, PSMAFGCP, PSMPteroylpoly-gamma-glutamate carboxypeptidase
• Conjugate: Biotin
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PSMA/FOLH1/NAALADase I