R&D Systems™ Recombinant Human PTEN Protein

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Inhibition Activity

Measured by its ability to dephosphorylate either diC8PIP3 or diC16PIP3. The specific activity is >480 nmol/min/mg, as measured under the described conditions.

Specifications

• Accession Number: P60484
• For Use With (Application): Inhibition Assay
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, EDTA, Glycerol and Betamercaptoethanol.
• Gene ID (Entrez): 5728
• Molecular Weight (g/mol): M.W. (Observed): 59 kDa, reducing conditions; M.W. (theoretical): 48 kDa
• Name: PTEN
• Quantity: 10 μg
• Source: E. coli-derived human PTEN protein Thr2-Val403, with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: PTEN
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >80%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PTEN