R&D Systems™ Recombinant Human PTP1B Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006 ). The specific activity is >15 μmol/min/mg, as measured under the described conditions.

Specifications

• Accession Number: P18031
• For Use With (Application): Enzyme Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and DTT.
• Gene ID (Entrez): 5770
• Molecular Weight (g/mol): M.W. (Observed): 38 kDa, reducing conditions; M.W. (theoretical): 38 kDa
• Name: PTP1B
• Quantity: 50 μg
• Source: E. coli-derived human PTP1B protein Glu2-Asn321, with an N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: PTPN1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute to 100 μg/mL in sterile 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 1 mM dithiothreitol, and 500 μg/mL bovine serum albumin, pH 7.5.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Protein: PTP1B/PTPN1