R&D Systems™ Recombinant Human R-Spondin 1 Biotinylated Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human R-Spondin 1 Biotinylated Protein is derived from CHO. The Recombinant Human R-Spondin 1 Biotinylated Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q2MKA7
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 284654
• Molecular Weight (g/mol): Theoretical M.W.: 26kDa (unlabeled)
• Quantity: 25μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human R-Spondin 1 protein Arg31-Ala263
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: Cristin 3, CRISTIN3, FLJ40906Roof plate-specific spondin-1, hRspo1, roof plate-specific spondin, RSPO, RSPO1, RSPONDIN, RSpondin 1, R-spondin homolog (Xenopus laevis), R-spondin1, R-spondin-1
• Common Name: R-Spondin 1
• Gene Symbol: RSPO1
• Biological Activity: Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 5-30ng/mL in the presence of 5ng/mL recombinant mouse Wnt-3a.
• Conjugate: Biotin
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Arg31-Ala263
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining