R&D Systems™ Recombinant Human R-Spondin 1 Biotinylated Protein

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human Lgr5/GPR49 Fc Chimera (Catalog # 8078-GP ) is immobilized at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human R-Spondin 1 (Catalog # BT4645B) binds with an ED50 of 4.50-54.0 ng/mL.

Specifications

• Accession Number: Q2MKA7.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 284654
• Molecular Weight (g/mol): MolecularWeight-observed: 29-42 kDa, under reducing conditions., MolecularWeight-theroretical: 25.6 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein Ser21-Ala263
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Cristin 3, CRISTIN3, FLJ40906Roof plate-specific spondin-1, hRspo1, roof plate-specific spondin, RSPO, RSPO1, RSPONDIN, RSpondin 1, R-spondin homolog (Xenopus laevis), R-spondin1, R-spondin-1
• Conjugate: Biotin
• Reconstitution: Reconstitute at 250 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: R-Spondin 1