R&D Systems™ Recombinant Human R-Spondin 3 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.500-2.00 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN ).

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 84870
• Molecular Weight (g/mol): 28.3 kDa
• Name: R-Spondin 3
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human R-Spondin 3 protein Gln22-His272
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.