R&D Systems™ Recombinant Human Ret His-tag Protein

Description

Measured by its binding ability in a functional ELISA.

When Recombinant Human Ret His-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Human GDNF binds with an ED50 of 0.03 to 0.3 μg/mL in the presence of Recombinant Human GFR alpha -1/GDNF R alpha-1 Fc Chimera

Specifications

• Accession Number: P07949
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 5979
• Molecular Weight (g/mol): 69 kDa
• Name: CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human Ret protein Leu29-Arg635, with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Endotoxin Level: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.