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R&D Systems™ Recombinant Human Ret His-tag Protein

Catalog No. 10302CR050
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50 μg
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10-302-CR050 50 μg
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Measured by its binding ability in a functional ELISA.

When Recombinant Human Ret His-tag is immobilized at 1 μg/mL (100 μL/well), Recombinant Human GDNF binds with an ED50 of 0.03 to 0.3 μg/mL in the presence of Recombinant Human GFR alpha -1/GDNF R alpha-1 Fc Chimera

Specifications

Accession Number P07949
For Use With (Application) Bioactivity
Gene ID (Entrez) 5979
Molecular Weight (g/mol) 69 kDa
Name CDHF12MTC1, CDHR16, EC 2.7.10, Hirschsprung disease 1, Hirschsprung disease), MEN2A, MEN2B, multiple endocrine neoplasia and medullary thyroid carcinoma 1, ret proto-oncogene, RET transforming sequence
Quantity 50 μg
Source Mouse myeloma cell line, NS0-derived human Ret protein Leu29-Arg635, with a C-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 500 μg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level <0.10 EU per 1 μg of the protein by the LAL method.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
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