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R&D Systems™ Recombinant Human ROBO1/DUTT1 Fc Chimera Protein

Catalog No. 8975RB050
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89-75R-B050 50μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human ROBO1/DUTT1 Fc Chimera Protein is derived from NS0. The Recombinant Human ROBO1/DUTT1 Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

For Use With (Application) Bioactivity
Gene ID (Entrez) 6091
Molecular Weight (g/mol) Theoretical M.W.: 119kDa
Quantity 50μg
Source Mouse myeloma cell line,NS0-derived human ROBO1 protein, (N-terminus) Human ROBO1/DUTT1 (Ser20-Pro858) Accession &Num; NP_598334.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias DUTT1, DUTT1Drosophila) homolog 1, H-Robo-1, MGC131599, MGC133277, roundabout, axon guidance receptor, homolog 1 (Drosophila)
Common Name ROBO1
Gene Symbol ROBO1
Biological Activity Measured by its binding ability in a functional ELISA. When recombinant human Slit3 is coated at 250ng/mL, Recombinant Human ROBO1 Fc Chimera binds with a typical ED50 of 100-600ng/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived Human ROBO1/DUTT1 (Ser20-Pro858) Accession # NP_598334.1, N-terminus; IEGRMD; Human IgG1 (Pro100-Lys330), C-terminus
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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