R&D Systems™ Recombinant Human SCF Protein

Description

Recombinant Protein

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1.00-5.00 ng/mL.

Specifications

• Accession Number: P21583.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 4254
• Molecular Weight (g/mol): MolecularWeight-observed: 22-31 kDa, under reducing conditions.; MolecularWeight-theroretical: 18 kDa
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human SCF/c-kit Ligand protein Glu26-Ala189
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: c-kit Ligand, DCUA, DFNA69, DKFZp686F2250, familial progressive hyperpigmentation 2, FPH2, FPHH, KIT ligand, Kitl, KITLG, KL-1, Mast cell growth factor, MGF, MGFSHEP7, SCFStem cell factor, SFc-Kit ligand, SHEP7, SLF, steel factor
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100-500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: SCF/c-kit Ligand