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R&D Systems™ Recombinant Human Semaphorin 3E Protein

Catalog No. 3239S3B025
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25 μg
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32-39S-3B025 25 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

The Recombinant Human Semaphorin 3E Protein is derived from NS0. The Recombinant Human Semaphorin 3E Protein has been validated for the following applications: Bioactivity.

Specifications

Accession Number O15041
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2μm filtered solution in PBS, NaCl and Tween™ 20 with Trehalose.
Gene ID (Entrez) 9723
Molecular Weight (g/mol) Observed M.W.: 85-100kDa, 66kDa and 25kDa, reducing conditions, Theoretical M.W.: 88kDa
Quantity 25 μg
Source Mouse myeloma cell line,NS0-derived human Semaphorin 3E protein His24-Ser775 (Arg557Ala and Arg560Ala),with an N-terminal 10-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <1.0 EU per 1μg of the protein by the LAL method.
Gene Alias (semaphorin) 3E, coll-5, KIAA0331M-SEMAH, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, Sema3E, SEMAH, SEMAHM-SemaK, semaphorin-3E
Common Name Semaphorin 3E
Gene Symbol SEMA3E
Biological Activity Measured by its ability to inhibit the proliferation of HUVEC human umbilical vein endothelial cells. Moriya, J. et al. (2010) Circ. Res. 106:391. The ED50 for this effect is typically 0.04-0.24μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin D1 is coated at 5μg/mL, Recombinant Human Semaphorin 3E binds with an apparent Kd <0.4 nM.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived His24-Ser775 (Arg557Ala and Arg560Ala), with an N-terminal 10-His tag
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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