R&D Systems™ Recombinant Human Semaphorin 4F Fc Chimera Protein

Catalog No.	Quantity
10-113-S4050	50 μg

Catalog No. 10-113-S4050 Supplier R&D Systems™ Supplier No. 10113S4050

Description

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.

Recombinant Human Semaphorin 4F Fc Chimera immobilized at 2.5 μg/mL on a 96 well plate and is able to significantly enhance neurite outgrowth

Specifications

• Accession Number: O95754-1
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 10505
• Molecular Weight (g/mol): 96 to 111 kDa, under reducing conditions
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 4F protein. Human Semaphorin 4F (Arg35-Val659) Accession # O95754-1IEGRMDHuman IgG1 (Pro100-Lys330)N-terminus C-terminus
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: < 0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 250 μg/mL in Water
• Buffer: Lyophilized from a 0.2 μm filtered solution in Citric Acid and NaCl
• Purity or Quality Grade: > 85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining