R&D Systems™ Recombinant Human Serpin B2/PAI-2 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Serpin B2/PAI-2 Protein is derived from E. coli. The Recombinant Human Serpin B2/PAI-2 Protein has been validated for the following applications: Enzyme Activity.

Specifications

• Accession Number: P05120
• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 5055
• Molecular Weight (g/mol): Observed M.W.: 41kDa, reducing conditions, Theoretical M.W.: 44kDa
• Quantity: 25 μg
• Source: E. coli-derived human Serpin B2 protein Glu2-Ala65/Ala99-Pro415,with an N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70° C as supplied. 3 months, -70° C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Gene Alias: HsT1201, Monocyte Arg-serpin, PAI, PAI2, PAI2Urokinase inhibitor, Placental plasminogen activator inhibitor, PLANH2, PLANH2PAI-2, plasminogen activator inhibitor 2, plasminogen activator inhibitor, type II (arginine-serpin), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2, Serpin B2, serpin peptidase inhibitor, clade B (ovalbumin), member 2, Urokinase Inhibitor
• Common Name: SerpinB2
• Biological Activity: Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The inhibition IC50 is <15 nM, as measured under the described conditions. See Activity Assay Protocol on RnDSystems.com.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Glu2-Ala65/Ala99-Pro415, with an N-terminal Met and 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining