R&D Systems™ Recombinant Human Siglec-15 Fc Chimera Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.

Order Info

The product is shipped at ambient temperature.

Specifications

• Accession Number: Q6ZMC9
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
• Gene ID (Entrez): 284266
• Molecular Weight (g/mol): M.W. (Observed): 53-64 kDa, reducing conditions; M.W. (theoretical): 53 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein Human Siglec-15 (Phe20-Thr263) (N-terminus) Accession # Q6ZMC9 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 μg/mL aq. soln..
• Activity: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Siglec-15

For Research Use Only