R&D Systems™ Recombinant Human Siglec-2/CD22 Fc Alexa Fluor™ 647 Protein

Description

Fc Chimera

Measured by flow cytometry for its ability to bind anti-human Siglec-2/CD22 Monoclonal Antibody conjugated beads.The concentration of Recombinant Human Siglec-2/CD22 Fc Chimera Alexa Fluor™ 647 (Catalog # AFR1968) that produces 50% of the binding response is 2.50-25.0 ng/mL

Specifications

• Accession Number: CAA42006.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 933
• Molecular Weight (g/mol): M.W.-observed: 120-132 kDa, under reducing conditions., M.W.-theroretical: 101.9 kDa (monomer)
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human Siglec-2/CD22 protein Human Siglec-2 (Asp20-Arg687) (N-terminus) Accession # CAA42006.1 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule, CD22, CD22 antigenMGC130020, CD22 molecule, sialic acid binding Ig-like lectin 2, Sialic acid-binding Ig-like lectin 2, Siglec2, SIGLEC-2, SIGLEC2FLJ22814, T-cell surface antigen Leu-14
• Conjugate: Alexa Fluor 647
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Siglec-2/CD22