R&D Systems™ Recombinant Human Siglec-3/CD33 Fc Alexa Fluor™ 647 Protein

Catalog No.	Quantity
AFR1137020	20 μg

Catalog No. AFR1137020 Supplier R&D Systems™ Supplier No. AFR1137020

Description

Recombinant Protein

Measured by flow cytometry for its ability to bind anti-human Siglec-3/CD33 Monoclonal Antibody conjugated beads.The concentration of Recombinant Human Siglec-3/CD33 Fc Chimera Alexa Fluor™ 647 (Catalog # AFR1137) that produces 50% of the binding response is 0.50-20.0 ng/mL.

Specifications

• Accession Number: AAA51948
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 945
• Molecular Weight (g/mol): M.W.-observed: 67-85 kDa, under reducing conditions, M.W.-theroretical: 53.4 kDa (monomer)
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human Siglec-3/CD33 protein Human Siglec-3 (Asp18-His259)(Val257Leu) (N-terminus) Accession # AAA51948 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD33, CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, gp67, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, Siglec3, SIGLEC-3, SIGLEC3gp67
• Conjugate: Alexa Fluor 647
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Siglec-3/CD33