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R&D Systems™ Recombinant Human Siglec-8 Fc Chimera Protein

Catalog No. 9045SL050
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90-45S-L050 50 μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Siglec-8 Fc Chimera Protein is derived from NS0. The Recombinant Human Siglec-8 Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

Accession Number AAH53319
For Use With (Application) Bioactivity
Gene ID (Entrez) 27181
Molecular Weight (g/mol) Observed M.W.: 68-88kDa, reducing conditions, Theoretical M.W.: 64kDa
Quantity 50 μg
Source Mouse myeloma cell line,NS0-derived human Siglec-8 protein, (N-terminus) Human Siglec-8 (Met17-Ala363) Accession &Num; AAH53319 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias CD329 antigen, CDw329, MGC59785, SAF2, SAF2SAF-2, sialic acid binding Ig-like lectin 8, Sialoadhesin family member 2, Siglec8, Siglec-8, SIGLEC8L, SIGLEC8Lsialic acid-binding Ig-like lectin 8
Common Name Siglec-8
Gene Symbol SIGLEC8
Biological Activity Measured by its binding ability in a functional ELISA. When Biotinylated Sialyl Lewis X was immobilized at 0.05μg/mL (100μL/well), it binds Recombinant Human Siglec-8 Fc Chimera. The concentration of Human Siglec-8 Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1.2μg/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Mouse myeloma cell line, NS0-derived Human Siglec-8 (Met17-Ala363) Accession # AAH53319, N-terminus; IEGRMD; Human IgG1 (Pro100-Lys330), C-terminus
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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