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R&D Systems™ Recombinant Human SIRP alpha/CD172a Alexa Fluor™ 488 Protein

Catalog No. AFG4546020
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20 μg
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AFG4546020 20 μg
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Fc Chimera

Measured by flow cytometry for its ability to bind Human SIRP alpha/CD172a Antibody conjugated fluorescent beads at 0.313-1.25 μg/mL (100 μL/well). Please Note: Optimal dilutions should be determined by each laboratory for each application.

Specifications

Accession Number NP_542970.1
For Use With (Application) Bioactivity
Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Gene ID (Entrez) 140885
Molecular Weight (g/mol) MolecularWeight-observed: 70-105 kDa, under reducing conditions., MolecularWeight-theroretical: 64 kDa (monomer)
Quantity 20 μg
Source Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Human SIRP alpha (Gly27-Arg370) & (Glu31-Arg370) (N-terminus) Accession # NP_542970.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <1.0 EU / 1 μg of the protein by the LAL method.
Gene Alias Bit, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MYD1, MYD-1, MyD-1 antigen, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRPA, SIRPalpha, SIRP-ALPHA-1, Sirp-alpha-2, SIRPalpha2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1
Conjugate Alexa Fluor 488
Purity or Quality Grade >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein SIRP alpha/CD172a
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