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R&D Systems™ Recombinant Human SKP1/SKP2 Complex Protein, CF

Catalog No. E3521025
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Quantity:
25 μg
This item is not returnable. View return policy
This item is not returnable. View return policy

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription

Typical protein concentration for use in vitro will depend on experimental conditions.

Specifications

For Use With (Application) Bioactivity
Formulation X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Molecular Weight (g/mol) M.W. (theoretical): 19 kDa (SKP1), 48 kDa (SKP2)
Purification Method >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
Quantity 25 μg
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/SKP2 Complex protein
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method
Conjugate Unconjugated
Buffer X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Purity or Quality Grade >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
Protein SKP1/SKP2 Complex
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