R&D Systems™ Recombinant Human SKP1/SKP2 Complex Protein, CF

Description

SKP1 (S-phase kinase-associated protein 1) is an essential component of SCF (SKP1-CUL1-F-box protein) E3 Ubiquitin ligases that mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription

Typical protein concentration for use in vitro will depend on experimental conditions.

Specifications

• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Molecular Weight (g/mol): M.W. (theoretical): 19 kDa (SKP1), 48 kDa (SKP2)
• Purification Method: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human SKP1/SKP2 Complex protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 36 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Buffer: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Purity or Quality Grade: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: SKP1/SKP2 Complex