R&D Systems™ Recombinant Human ST3GAL1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to bovine B1-3 galactosyl-N-acetyl galactosamine. The specific activity is >1000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: Q11201
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 6482
• Molecular Weight (g/mol): M.W. (Observed): 35-45 kDa, reducing conditions; M.W. (theoretical): 33.4 kDa
• Name: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 protein Arg57-Arg340, with an N-terminal HA (YPYDVPDYA) tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: ST3GAL1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A