R&D Systems™ Recombinant Human TGF-beta 1, ACFP Protein

Description

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells

• ED50 for this effect is 0.04 to 0.2 ng/mL.
• Specific activity of recombinant human TGF-beta 1 is approximately 2.5 x 104 U/μg, which is calibrated against human TGF-beta 1 Standard (NIBSC code: 89/514).

Specifications

• Accession Number: P01137
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7040
• Molecular Weight (g/mol): 11 kDa, reducing conditions
• Quantity: 10 μg
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human TGF-beta 1 protein Ala279-Ser390 Produced in an animal component free process (ACFP)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: < 0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile 4 mM HCl
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA
• Purity or Quality Grade: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining