R&D Systems™ Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF

Description

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.200 ng/mL.

Specifications

• Accession Number: P01137.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Gene ID (Entrez): 7040
• Molecular Weight (g/mol): M.W. (Observed): 12 kDa, under reducing conditions & 24 kDa under non-reducing conditions., M.W. (Theroretical): 13 kDa
• Quantity: 500 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CEDLAP, DPD1, latency-associated peptide, TGF beta, TGFB, TGFbeta, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 10 μg size at 100 μg/mL in 4 mM HCl. Reconstitute all other sizes at 250 μg/mL in 4 mM HCl.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: TGF-beta 1