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R&D Systems™ Recombinant Human TGF-beta 1 (CHO-Expressed) Protein, CF

Catalog No. 11409BH500
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Catalog No. Quantity
11-409-BH500 500 μg
11-409-BH100 100 μg
11-409-BH01M 1 mg
11-409-BH010 10 μg
11-409-BH050 50 μg
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Catalog No. 11-409-BH500 Supplier R&D Systems™ Supplier No. 11409BH500
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Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.200 ng/mL.

Specifications

Accession Number P01137.2
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
Gene ID (Entrez) 7040
Molecular Weight (g/mol) M.W. (Observed): 12 kDa, under reducing conditions & 24 kDa under non-reducing conditions., M.W. (Theroretical): 13 kDa
Quantity 500 μg
Source Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1 μg of the protein by the LAL method.
Gene Alias CEDLAP, DPD1, latency-associated peptide, TGF beta, TGFB, TGFbeta, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1
Conjugate Unconjugated
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in 4 mM HCl. Reconstitute all other sizes at 250 μg/mL in 4 mM HCl.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein TGF-beta 1
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