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R&D Systems™ Recombinant Human TGF-beta 1 Protein

Catalog No. 240B002
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Quantity:
1 mg
10 μg
2 μg
Formulation:
Lyophilized from a 0.2 μm filtered solution in acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT-2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.

Specifications

Accession Number P01137
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in acetonitrile and TFA with BSA as a carrier protein.
Gene ID (Entrez) 7040
Molecular Weight (g/mol) M.W. (Observed): 12 kDa, reducing conditions 24 kDa, non-reducing conditions; M.W. (theoretical): 12.8 kDa (monomer)
Name TGF-beta 1
Quantity 2 μg
Source Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein Ala279-Ser390
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CEDLAP, DPD1, latency-associated peptide, TGF beta1, TGFB, TGFB1, TGFbeta, TGFbeta 1, TGF-beta 1 protein, TGF-beta-1, transforming growth factor beta-1, transforming growth factor, beta 1
Gene Symbol TGFB1
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces. To ensure recovery, reconstitute 2 μg vials at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin. Reconstitute 10 μg or larger vials at 100 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.
Purity or Quality Grade >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein TGF-beta 1
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