R&D Systems™ Recombinant Human Thioredoxin 80 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to induce IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC) in the presence of IL-2. Pekkari, K. et al. (2001) Blood 97:3184. The ED50 for this effect is 0.150-1.50 μg/mL in the presence of 8 ng/mL of recombinant human IL-2.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in Tris, EDTA and DTT.
• Gene ID (Entrez): 7295
• Molecular Weight (g/mol): 9 kDa (monomer)
• Name: Thioredoxin-80
• Quantity: 100 μg
• Source: E. coli-derived human Thioredoxin-80 protein Val2-Lys81
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Structural Form: Noncovalently-linked homodimer
• Endotoxin Concentration: <0.50 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.