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R&D Systems™ Recombinant Human Thrombopoietin His (HEK-293) Protein

Catalog No. 288TPH500
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Quantity:
1 mg
10 μg
100 μg
50 μg
500 μg
This item is not returnable. View return policy
This item is not returnable. View return policy

Analyzed by SEC-MALS. His-tag (HEK-293-expressed)

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.0500-0.500 ng/mL.

Specifications

Accession Number P40225.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 7066
Molecular Weight (g/mol) MolecularWeight-observed: 26-30 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa
Quantity 500 μg
Source Human embryonic kidney cell, HEK293-derived human Thrombopoietin/Tpo protein Ser22-Leu195, with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF
Conjugate Unconjugated
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes (at 500 μg/mL in PBS.)
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Thrombopoietin/THPO
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