R&D Systems™ Recombinant Human TNF-alpha Avi-tag Protein

Catalog No.	Quantity
AVI10728025	25 μg

Catalog No. AVI10728025 Supplier R&D Systems™ Supplier No. AVI10728025

Description

Biotinylated

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TNF RI/TNFRSF1A Fc Chimera (Catalog # 372-RI) is coated at 0.025 μg/mL (100 μL/well), Biotinylated Recombinant Human TNF-alpha Avi-tag binds with an ED50 of 2-12 ng/mL. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 20-200 pg/mL.

Specifications

• Accession Number: P01375.1
• For Use With (Application): Bioactivity, Gel Filtration Chromatography
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 7124
• Molecular Weight (g/mol): M.W.-observed: 15-22 kDa, under reducing conditions, M.W.-theroretical: 19 kDa
• Quantity: 25 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human TNF-alpha protein Avi-tag Human TNF alpha (Val77-Leu233) (c-terminus) Accession # P01375.1 N-terminusC-terminμs
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: TNF-alpha