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R&D Systems™ Recombinant Human TNF RII/TNFRSF1B Fc Avi-tag Protein

Catalog No. AVI10911050
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50 μg
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AVI10911050 50 μg
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Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human TNF alpha (Catalog # 210-TA ) is coated at 0.100 μg/mL (100 μL/well), Biotinylated Recombinant Human TNF RII/TNFRSF1B Fc Chimra Avi-tag binds with an ED50 of 4.00-24.0 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Specifications

Accession Number AAA36755.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 7133
Molecular Weight (g/mol) M.W.-observed: 68-81 kDa, under reducing conditions, M.W.-theroretical: 54 kDa
Quantity 50 μg
Source Human embryonic kidney cell, HEK293-derived human TNF RII/TNFRSF1B protein Human TNF RII/TNFRSF1B (Leu23-Asp257) (N-terminus) Accession #AAA36755.1 IEGRMD Human IgG1 (Pro100-Lys330) Avi-tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CD120b, CD120b antigen, Etanercept, p75 TNF receptor, p75TBPII, p75TNFR, soluble TNFR1B variant 1, TNFBRp80 TNF-alpha receptor, TNF-R2, TNFR2TNFR1B, TNF-R75, TNFR80, TNFRII, TNF-RII, TNF-R-II, TNFR-II, TNFRSF1B, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, Tumor necrosis factor receptor 2, tumor necrosis factor receptor superfamily member 1 B, tumor necrosis factor receptor superfamily, member 1 B, Tumor necrosis factor receptor type II
Conjugate Biotin
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Protein TNF RII/TNFRSF1B
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