R&D Systems™ Recombinant Human u-Plasminogen Activator/Urokinase Avi, CF

Catalog No.
AVI1310020	20 μg

Catalog No. AVI1310020 Supplier R&D Systems™ Supplier No. AVI1310020

Description

His-tag Avi-tag

Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human u-Plasminogen Activator (uPA)/Urokinase His-tag Avi-tag (Catalog # AVI1310) binds to Human u-Plasminogen Activator (uPA) Antibody (Catalog # MAB1310 ) with an ED50 of 0.300-4.50 ng/mL.

Specifications

• Accession Number: P00749.2
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl and CaCl2.
• Gene ID (Entrez): 5328
• Molecular Weight (g/mol): MolecularWeight-observed: 19-20 kDa & 32-38 kDa, under reducing conditions., MolecularWeight-theroretical: 18 kDa (long A chain), 3 kDa (short A chain), 31 kDa (B chain)
• Quantity: 20 μg
• Source: Human embryonic kidney cell, HEK293-derived human u-Plasminogen Activator (uPA)/Urokinase protein Met1-Leu431 with C-terminal 6-His and Avi-tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: ATF, EC 3.4.21, EC 3.4.21.73, plasminogen activator, urokinase, PLAU, uPA, u-PA, uPlasminogen Activator, U-plasminogen activator, urinary, Urokinase, urokinase-type plasminogen activator
• Conjugate: Biotin
• Protein: u-Plasminogen Activator/Urokinase