R&D Systems™ Recombinant Human UbcH5a/UBE2D1 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.

Specifications

• Accession Number: P51668.1
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
• Gene ID (Entrez): 7321
• Molecular Weight (g/mol): M.W. (theoretical): 17 kDa
• Name: UbcH5a/UBE2D1
• Quantity: 100 μg
• Source: E. coli-derived human UbcH5a/UBE2D1 protein Met1 - Met147
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: UBE2D1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: UbcH5a/UBE2D1