R&D Systems™ Recombinant Human UBE2I/Ubc9 Protein

Catalog No.	Quantity
E2645100	100 μg

Catalog No. E2645100 Supplier R&D Systems™ Supplier No. E2645100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human UBE2I/Ubc9 is a member of the SUMO-conjugating (E2) enzyme family that receives SUMO from a SUMO-activating (E1) enzyme and subsequently conjugates SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UBE2I/Ubc9 concentration of 0.1-1 μM.

Specifications

• Accession Number: P63279
• For Use With (Application): Enzyme Activity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
• Gene ID (Entrez): 7329
• Molecular Weight (g/mol): M.W. (theoretical): 18 kDa
• Name: UBE2I/Ubc9
• Quantity: 100 μg
• Source: E. coli-derived human UBE2I/Ubc9 protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: UBE2I
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: UBE2I/Ubc9