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R&D Systems™ Recombinant Human VEGF 165, ACFP Protein

Catalog No. ACFP29301M
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ACFP29301M 1 mg
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Catalog No. ACFP29301M Supplier R&D Systems™ Supplier No. ACFP29301M

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Description

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.

  • ED50 for this effect is 1 to 6 ng/mL.
  • Specific activity of recombinant human VEGF is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF 165 WHO Standard (NIBSC code: 02/286).

Specifications

Accession Number NP_001165097
For Use With (Application) Bioactivity
Gene ID (Entrez) 7422
Molecular Weight (g/mol) 19 to 21 kDa, reducing conditions
Quantity 1 mg
Source Spodoptera frugiperda, Sf 9 (baculovirus)-derived human VEGF protein Met1-Arg191 Produced in an animal component free process (ACFP)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Endotoxin Concentration < 0.01 EU per 1 μg of the protein by the LAL method
Conjugate Unconjugated
Species Human
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. Alternatively, reconstitute (at 100-500 μg/mL in sterile 4 mM HCl.)
Buffer Lyophilized from a 0.2 μm filtered solution in HCl
Purity or Quality Grade > 97%, by SDS-PAGE under reducing conditions and visualized by silver Stain
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