R&D Systems™ Recombinant Human VEGF 165 (E. coli-expressed) Protein, CF

Catalog No.	Quantity
10-318-VE010	10 μg

Catalog No. 10-318-VE010 Supplier R&D Systems™ Supplier No. 10318VE010

Description

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells.

Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-8 ng/mL. The specific activity of Recombinant Human VEGF 165 (Catalog # 10318-VE) is approximately 1.1 x 103 U/μg, which is calibrated against recombinant human VEGF 165 WHO Standard (NIBSC code: 02/286).

Specifications

• Accession Number: NP_001165097
• Formulation: Lyophilized from a 0.2 μm filtered solution in HCl.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): observed_molecular_weight: 19-22 kDa, under reducing conditions.
• Quantity: 10 μg
• Source: E. coli-derived human VEGF protein Ala27-Arg191
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS. Alternatively, reconstitute (at 100-500 μg/mL in sterile 4 mM HCl..)
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: VEGF