R&D Systems™ Recombinant Human VEGF 165 (HEK293-expressed) Protein, CF

Description

Recombinant Protein

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL.

Specifications

• Accession Number: NP_001165097.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): MolecularWeight-observed: 19-25 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa
• Quantity: 500 μg
• Source: Human embryonic kidney cell, HEK293-derived human VEGF protein Ala27-Arg191
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 10 μg size at 100 μg/mL in 4mM HCl. Reconstitute all other sizes at 500 μg/mL in 4mM HCl.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VEGF