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R&D Systems™ Recombinant Human VEGF 165 Protein

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Quantity:
1 mg
10 μg
50 μg
500 μg
Formulation:
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with trehalose.
Description
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286). Specific activity is for reference purposes only and is not routinely tested.
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Specifications
Specifications
Formulation | Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Gene ID (Entrez) | 7422 |
Molecular Weight (g/mol) | observed_molecular_weight: 20-22 kDa, under reducing conditions. 39-42 kDa, under non-reducing conditions. |
Name | VEGF 165 |
Quantity | 50 μg |
Source | Spodoptera frugiperda,Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191 |
Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. |
Structural Form | Disulfide-linked homodimer |
Conjugate | Unconjugated |
Recombinant | Recombinant |
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