R&D Systems Recombinant Human VEGF 165 Protein
R&D Systems Recombinant Human VEGF 165 Protein stimulates proliferation in HUVEC human umbilical vein endothelial cells.
Manufacturer: R&D Systems 293VE01M
DescriptionMeasured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED 50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF 165 is approximately 1.7 x 10 3 U/µg, which is calibrated against recombinant human VEGF 165 WHO Standard (NIBSC code: 02/286).
|>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor|
|Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.|
|<0.01EU per 1μg of the protein by the LAL method.|
|Reconstitute at 100μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.|
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