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R&D Systems™ Recombinant Human VEGF 165 Protein

R&D Systems™ Recombinant Human VEGF 165 Protein stimulates proliferation in HUVEC human umbilical vein endothelial cells.

Manufacturer:  R&D Systems™ 293VE01M

Catalog No. 29-3VE-01M


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Description

Description

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED 50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF 165 is approximately 1.7 x 10 3 U/µg, which is calibrated against recombinant human VEGF 165 WHO Standard (NIBSC code: 02/286).
Specifications

Specifications

NP_001165097
Bioactivity
7422
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
Recombinant Protein
Human
Bioactivity
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
19.2kDa (monomer)
1mg
<0.01EU per 1μg of the protein by the LAL method.
VEGFA
Unconjugated
Reconstitute at 100μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.
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