R&D Systems™ Recombinant Human VEGF 165 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286). Specific activity is for reference purposes only and is not routinely tested.

Specifications

• Accession Number: NP_001165097
• Characterization: Bioactivity
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): observed_molecular_weight: 20-22 kDa, under reducing conditions. 39-42 kDa, under non-reducing conditions.
• Quantity: 1 mg
• Source: Spodoptera frugiperda,Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01EU per 1μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Gene Symbol: VEGFA
• Product Type: Recombinant Protein
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute (at 100-500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.)
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.