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R&D Systems™ Recombinant Human VEGF 165 Protein, Animal-Free
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Quantity:
1 mg
50 μg
Description
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL.The specific activity of recombinant human VEGF165 is >8.0 x 105 units/mg, which is calibrated against the human VEGF165 WHO standard (NIBSC code: 02/286).
Specifications
Specifications
Accession Number | NP_001165097.1 |
For Use With (Application) | Bioactivity |
Formulation | Lyophilized from a 0.2 μm filtered solution in Sodium Acetate. |
Gene ID (Entrez) | 7422 |
Molecular Weight (g/mol) | M.W.-observed: 19-21 kDa, under reducing conditions., M.W.-theroretical: 19.2 kDa (monomer) |
Quantity | 1 mg |
Source | E. coli-derived human VEGF protein Ala27-Arg191 Produced using non-animal reagents in an animal-free laboratory. |
Storage Requirements | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. |
Endotoxin Concentration | <0.01 EU / 1 μg of the protein by the LAL method. |
Gene Alias | MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor |
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