R&D Systems™ Recombinant Human VEGF 165 Protein, Animal-Free

Description

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1.50-12.0 ng/mL.The specific activity of recombinant human VEGF165 is >8.0 x 105 units/mg, which is calibrated against the human VEGF165 WHO standard (NIBSC code: 02/286).

Specifications

• Accession Number: NP_001165097.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): M.W.-observed: 19-21 kDa, under reducing conditions., M.W.-theroretical: 19.2 kDa (monomer)
• Quantity: 1 mg
• Source: E. coli-derived human VEGF protein Ala27-Arg191 Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile deionized water.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VEGF