R&D Systems™ Recombinant Human VEGF-Ax Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human VEGF-Ax Protein is derived from CHO. The Recombinant Human VEGF-Ax Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: NP_001303939
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): Observed M.W.: 22-29kDa, reducing conditions, Theoretical M.W.: 21kDakDa
• Quantity: 25μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human VEGF protein, (N-terminus) Human VEGF-A (Ala27-Arg191) Accession &Num; NP_001303939 SAGQEEGASLRVSGTRSLTRKD (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: MVCD1, VAS, vascular endothelial growth factor A, Vascular permeability factor, Vasculotropin, VEGFA, VEGF-A, VEGFMGC70609, VPF, VPFvascular endothelial growth factor
• Common Name: VEGF
• Gene Symbol: VEGFA
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera is immobilized at 0.5μg/mL (100μL/well), the concentration of Recombinant Human VEGF-Ax that produces 50% of the optimal binding response is approximately 1-6ng/mL. Recombinant Human VEGF-Ax weakly binds to Recombinant Human Neuropilin-1.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Human VEGF-A (Ala27-Arg191) Accession # NP_001303939, N-terminus; SAGQEEGASLRVSGTRSLTRKD, C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining