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R&D Systems™ Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein

Catalog No. 9057B7100
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100 μg
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90-57B-7100 100 μg
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Catalog No. 90-57B-7100 Supplier R&D Systems™ Supplier No. 9057B7100
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

Specifications

Accession Number Q9H7M9
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 64115
Molecular Weight (g/mol) M.W. (Observed): 30-65 kDa, reducing conditions; M.W. (theoretical): 19 kDa
Quantity 100 μg
Source Mouse myeloma cell line, NS0-derived human VISTA/B7-H5/PD-1 H protein Phe33-Ala194, with an Asp187Glu substitution and a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias 4632428N05Rik, B7H5, B7-H5, C10orf54, chromosome 10 open reading frame 54, Dies1, Gi24, PD1H, PD-1H, platelet receptor Gi24, PP2135, SISP1, stress induced secreted protein 1
Common Name VISTA/B7-H5/PD-1H
Gene Symbol C10orf54
Biological Activity Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is typically 0.5-3μg/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Reconstitution Reconstitute at 200 μg/mL in PBS.
Expression System Mouse myeloma cell line, NS0-derived Phe33-Ala194, with an Asp187Glu substitution and a C-terminal 6-His tag
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein VISTA/B7-H5/PD-1 H
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