R&D Systems™ Recombinant Human VSIG3 Fc Chimera Biotinylated Protein

Catalog No.	Quantity
BT-922-9050	50 μg

Catalog No. BT-922-9050 Supplier R&D Systems™ Supplier No. BT9229050

Description

Measured by its binding ability in a functional ELISA.

Measured by its binding ability in a functional ELISA. When Recombinant Human VISTA/B7-H5/PD-1H Fc Chimera Protein (Catalog # 7126-B7 ) is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human VSIG3 Fc Chimera Protein (Catalog # BT9229) that produces 50% of the optimal binding response is found to be approximately 2-10 μg/mL. Measured by its ability to inhibit anti-CD3 antibody induced IL-17 or IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 2-10 μg/mL.

Specifications

• Accession Number: BAC07546.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 152404
• Molecular Weight (g/mol): M.W. (Observed): 56-69 kDa, under reducing conditions; M.W. (theoretical): 50 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human VSIG3 protein Human VSIG3 (Leu23-Gly245) (N-terminus) Accession # BAC07546.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: VSIG3/IGSF11