R&D Systems™ Recombinant Human Wnt-1/sFRP-1 Complex Protein

Catalog No.	Quantity
11160WN010	10 μg

Catalog No. 11160WN010 Supplier R&D Systems™ Supplier No. 11160WN010

Description

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 25.0-250 ng/mL.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
• Gene ID (Entrez): 7471
• Molecular Weight (g/mol): M.W.-observed: 34-58 kDa, under reducing conditions., M.W.-theroretical: 38 kDa (Wnt-1) & 33 kDa (sFRP-1)
• Quantity: 10 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt-1 protein Human Wnt-1 (Ala28-Leu370) (C-terminus) Accession # P04628.1 Human sFRP-1 (Ser32-Lys314) (C-terminus) Accession # AAB70793.1 (N-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: FrzA, Int-1, INT1Proto-oncogene Int-1 homolog, proto-oncogene Wnt-1, SARP-2, sFRP1, wingless-type MMTV integration site family, member 1, wingless-type MMTV integration site family, member 1 (oncogene INT1), Wnt1
• Conjugate: Unconjugated
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: Wnt-1