R&D Systems™ Recombinant Human Wnt-3a GMP Protein, CF

Catalog No.	Quantity
50-36G-MP050	50 μg
50-36G-MP010	10 μg

Catalog No. 50-36G-MP050 Supplier R&D Systems™ Supplier No. 5036GMP050

Description

Recombinant Protein

Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is ≤ 25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.

Specifications

• Accession Number: NP_149122.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
• Gene ID (Entrez): 89780
• Molecular Weight (g/mol): Observed: 40 kDa, reducing conditions, Target: 37.4 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Wnt-3a protein Ser19-Lys352 Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
• Regulatory Status: GMP
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: MGC119418, MGC119419, MGC119420, protein Wnt-3a, wingless-type MMTV integration site family, member 3A, Wnt3a
• Gene Symbol: WNT3A
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Product Line: R&D Systems
• Purity or Quality Grade: >75%, by SDS-PAGE with silver staining, under reducing conditions
• Protein: Wnt-3a